You Are Interested in Cloning a Fragment from the Genome

question 97

Multiple Choice

You are interested in cloning a fragment from the genome of an organism where the G+C content of the sequence is 50%. The gene you wish to study is 2 kb (kilobase, each kb is 1000 bases) in length and does not include cleavage sites for any of the restriction enzymes below. For convenience in later experimental procedures, you want the average size of the fragments produced by the restriction enzyme to be approximately 4 kb. Which of the following restriction enzyme(s) would you choose to digest your genomic DNA?

• A)AluI
• B)EcoRI
• C)NotI
• D)Swa-I

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Definitions:

Mental Accounting

The psychological process of categorizing money into different accounts based on subjective criteria, affecting spending and investment decisions.

Behavioral Economics

A field of economic research that combines psychological insights with economic theory to understand how people make decisions and how they can sometimes deviate from rational decision-making.

Threat Of Rejection

The potential or perceived risk of having an offering, proposal, or application denied or refused.

Time Inconsistency

The tendency for people to change their plans about future actions due to a lack of alignment between their short-term desires and long-term goals.