You Are Interested in Studying Human ß-Globin and Decide to Produce

question 59

Multiple Choice

You are interested in studying human ß-globin and decide to produce the protein in bacterial cells. The ß-globin gene is a relatively simple gene with a single intron. You insert the entire gene, including the gene's promoter, in a plasmid and transform the recombinant plasmid into E. coli. You are disappointed to discover that your bacterial culture does not produce the correct protein. What are some possible explanations?

• A)The bacterial cell is missing RNA polymerase.
• B)The bacterial cell cannot splice introns.
• C)The bacterial cell does not recognize the eukaryote promoter.
• D)The bacterial cell produces the protein but it is rapidly degraded.

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