A Graduate Student Wants to Create a Recombinant DNA Molecule

question 90

Multiple Choice

A graduate student wants to create a recombinant DNA molecule and introduce this molecule into bacteria. What is the CORRECT order of steps that he should follow?

• A)choose plasmid/donor DNA  cut with restriction enzymes  join fragments via DNA ligase  transform bacteria
• B)choose plasmid  cut with restriction enzymes  join fragments with DNA ligase  introduce donor DNA  transform bacteria
• C)join plasmid and donor DNA using DNA ligase  cut with restriction enzymes  transform bacteria
• D)transform bacteria  choose plasmid/donor DNA  join fragments with DNA ligase
• E)cut plasmid/donor DNA with restriction enzymes  transform bacteria  isolate donor DNA/plasmid  join fragments with DNA ligase

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Definitions:

Crustal Thickness

The measure of the thickness of Earth's crust, which varies between the oceanic and continental crust.

Volcanism

the process through which magma and volcanic gases are expelled onto the Earth's surface, forming volcanic features.

Foreland Basin

A basin that forms when crust (either continental or oceanic) is warped by the weight of thrust sheets, especially when formed between a mountain belt and continental interior.

Passive Margin

A tectonically inactive edge of a continent, characterized by a transition from continental to oceanic lithosphere without significant seismic activity.