Bradykinin Is a Nonapeptide Released by Globulins in the Blood

question 26

Short Answer

Bradykinin is a nonapeptide released by globulins in the blood in response to a wasp sting. Hydrolysis gives the following amino acids: 2 Arg, Gly, 2 Phe, 3 Pro, and Ser. Edman degradation gives phenylthiohydantoin of Arg. Cleavage with carboxypeptidase gives Arg. Partial hydrolysis gives the following di- and tripeptides: Phe-Ser, Pro-Gly-Phe, Pro-Pro, Ser-Pro-Phe, Phe-Arg, and Arg-Pro. What is the amino acid sequence of bradykinin?

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