The CASPR/Cas9 System Is a New, Very Powerful Technique That

question 12

Multiple Choice

The CASPR/Cas9 system is a new, very powerful technique that can knockout genes in mice, flies, and animals that are not accessible to genetic manipulation. If you are trying to remove expression of a presynaptic protein you need to add a guide RNA and Cas9. What does the Cas9 do?

• A) Cas9 targets and removes the gene of interest by annealing the flanking regions.
• B) Cas9 binds to the gene of interest so that it is targeted for degradation by CASPR.
• C) Cas9 creates a double-stranded break in the DNA.
• D) Cas9 causes homologous recombination of sites around the targeted gene.

---

Definitions:

Temporalis

A broad, fan-shaped muscle on each side of the head that is involved in chewing and helps to close the jaw.

Vocalis

The muscle located within the vocal folds that adjusts tension, playing a key role in producing speech sounds by controlling pitch and tone.

Masseter

A facial muscle that plays a major role in the chewing process by elevating the mandible (jawbone).

Zygomaticus Major

A facial muscle that draws the angle of the mouth upward and outward, primarily involved in smiling.