The CASPR/Cas9 System Is a New, Very Powerful Technique That

question 33

Multiple Choice

The CASPR/Cas9 system is a new, very powerful technique that can knockout genes in mice, flies, and animals that are not accessible to genetic manipulation. If you are trying to remove expression of a presynaptic protein you need to add a guide RNA and Cas9. Why do you need a guide RNA? Choose all that apply.

• A) The guide RNA guides the Cas9 to the loxP site on the target DNA.
• B) The guide RNA targets the selected DNA and brings Cas9 to the DNA target site.
• C) The guide RNA guides CRISPR to the Cas9 protein to target the selected piece of DNA.
• D) CRISPR is inserted into the genome where the guide RNA guides Cas9 to the deletion site.

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Definitions:

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