What Is the Principle of Restriction Enzyme Analysis of Chromosomal

question 1

Multiple Choice

What is the principle of restriction enzyme analysis of chromosomal DNA?

• A) Plasmids are cut into small pieces with restriction enzymes. The resulting restriction fragment length polymorphism (RFLP) pattern is analyzed by agarose gel electrophoresis; there is transfer to a membrane, then use of a probe to identify specific sequences.
• B) The chromosomal DNA is denatured, then annealed. The resulting hybrids are electrophoresed, transferred to a membrane, and then analyzed for a specific sequence.
• C) DNA is extracted and isolated, and digested with a restriction enzyme. The resulting RFLP pattern is analyzed by agarose gel electrophoresis and transferred to a membrane.
• D) The serum specimen is electrophoresed, transferred to a membrane, digested, and then reacted with a probe to identify the target.

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