During the Hybridization/annealing Phase of a PCR Reaction,the Primers Bind

question 14

Multiple Choice

During the hybridization/annealing phase of a PCR reaction,the primers bind to the target DNA sequence at a specific temperature.What effect would an increase in the GC content of the primer have on the optimal temperature for annealing the primer to a target DNA sequence?

• A)the annealing temperature would increase
• B)the annealing temperature would decrease
• C)the annealing temperature would remain the same

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