A PCR Was Designed to Clone a DNA Sequence of 1.5

question 13

Multiple Choice

A PCR was designed to clone a DNA sequence of 1.5 kb.When the products were run on an agarose gel,there was a smear of bands ranging from 1.5 kb to 6 kb.Which of the following is a reason for this result?

• A)one of the primers did not bind
• B)the thermocycler malfunctioned
• C)the polymerase overran the reverse primer
• D)the polymerase denatured

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