Next- Generation Sequencing Takes Advantage of the Concept of Sequencing-

question 38

Multiple Choice

Next- generation sequencing takes advantage of the concept of sequencing- by- synthesis.What is sequence- by- synthesis?

• A)The use of PCR to incorporate ddNTPs,followed by electrophoresis.
• B)The use of a multiwell system and microfluidics to detect incorporated nucleotides at each base.
• C)The use of firefly luciferase and pyrophosphate to report on nucleotide incorporation.
• D)The use of a single polymerase in a nanopore that reports on a single molecule of synthesized DNA.

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Definitions:

Hydrochloric Acid

A digestive juice found in the stomach.

Stomach

An organ in the digestive system that breaks down food using acids and enzymes, preparing it for further digestion.

Acid Base

Refers to the concepts and processes related to acidic and basic properties of substances, crucial in chemical reactions, biology, and medicine for maintaining physiological pH balance.

Nitric Acid

a highly corrosive mineral acid used in the production of fertilizers, explosives, and in various industrial processes.