With Respect to Nucleic Acid Hybridization, Which, If Any, of the Following

question 6

Multiple Choice

With respect to nucleic acid hybridization, which, if any, of the following statements is false?

• A) The strength of base pairing between a probe and a complementary target sequence depends on the number of stable base pairs that are formed.
• B) Among other parameters, the hybridization stringency depends on the salt concentration and temperature of the hybridization reaction.
• C) To identify a target sequence that is distantly related to the probe, high stringency hybridization needs to be used.
• D) Under conditions that favor low hybridization stringency long heteroduplexes with significant base mismatching may be stable.

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Definitions:

Test Statistic

A value computed from sample data to decide whether to reject the null hypothesis in hypothesis testing.

Red Blood Cell Counts

A measurement of the number of red blood cells per unit volume of blood, used to assess overall health and diagnose various medical conditions.

Confidence Interval

A range of values, derived from sample statistics, that is likely to contain the value of an unknown population parameter, with a given level of confidence.

Ethanol

A volatile, flammable, colorless liquid commonly used as a fuel and in chemical synthesis, and also found in alcoholic beverages.