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question 7

Essay

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In cell-based DNA cloning a key step is ____ 1____ , the stage when the DNA of interest enters the provided host dells. The ____1______ efficiency is usually very low, but when ____ 1____ occurs just a ___2____ DNA molecule usually enters the cell. As a result, a complex starting DNA population can be fractionated by the cells (which effectively act as sorting offices). A second key step allows identification of ____3_____ cells (in the case of bacteria, the host cells are genetically modified to be sensitive to some ____4____ , and the vector carries a gene conferring ____5____ to the ____4____ ) . Many of the transformed cells contain just the vector DNA instead of the desired ____5____ DNA. To identify a specific ____5____ DNA, a more specific assay is required that often involves ____6_____ using a closely related labelled DNA or RNA ____7____.

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Definitions:

Cognitive Explanation

An approach in psychology that focuses on understanding the mental processes that underlie behavior and decision-making.

Contextual Explanation

An approach to understanding phenomena by considering the broader circumstances and factors that influence them, rather than isolating them from their environment.

Biological Explanation

An explanation of phenomena or behaviors based on biological factors, such as genetics, biochemistry, or physiology.

Sex-atypical Behavior

Actions or behaviors that do not conform to the traditional expectations of one's sex, often regarding interests, hobbies, or mannerisms.