In a Single PCR Cycle Consisting of 15 Seconds at 94°C

question 39

Multiple Choice

In a single PCR cycle consisting of 15 seconds at 94°C, 30 seconds at 50°C, and 1 min at 72°C, what is happening in the step run at 50°C?

• A) DNA polymerase is being inactivated.
• B) DNA polymerase is extending new DNA from the primers.
• C) Primers are being denatured.
• D) Primers are annealing to the DNA to be amplified.
• E) The DNA to be amplified is being denatured.

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Definitions:

Chi-Squared Curve

A graphical representation of the chi-squared distribution, showing the probability of chi-squared values along the x-axis.

Degrees of Freedom

A statistical concept that defines the number of independent pieces of information in a dataset used in parameter estimates.

Population Variances

The measurement of how much the numbers in a specific population are spread out from their mean.

Test Statistic

A value calculated from sample data that is used in hypothesis testing to determine whether to reject the null hypothesis.