Examlex
A culture of insulin-responsive cells are analyzed after stimulation with insulin.The insulin receptors are then isolated (assume that during the isolation protocol the insulin is removed from the receptor).Half of the sample is analyzed by SDS-PAGE followed by Coomassie blue staining.The other half of the sample is analyzed via SDS-PAGE followed by a Western blot.The primary antibody used in the blot recognizes phosphorylated tyrosine residues.Predict how many bands will be seen on the SDS-PAGE compared with the Western blot and explain your answer.
Interaction
The effect of two or more variables is not simply additive, but depends on the levels of the other variables.
Factorial ANOVA
A statistical test used to examine the effects of two or more independent variables on a dependent variable, and their interactions, within an experimental design.
Probability Level
The threshold of significance in hypothesis testing, indicating the likelihood that a result is not due to chance.
Main Effect
The direct influence of an independent variable on a dependent variable in an experiment, ignoring other factors or interactions.
Q5: An in vitro study shows that citrate
Q10: In the chloroplasts, photosystem I (PSI) and
Q15: Which of the following directly requires ATP
Q23: How does the directionality of synthesis differ
Q43: Some breast cancers express estrogen receptors and
Q50: The part of the Western blot that
Q71: If the titration curve shown below is
Q76: Below are the steps of a Western
Q79: The anaplerotic reaction catalyzed by pyruvate carboxylase
Q81: Compare the glycosyltransferase enzyme expression that results