Below Are the Steps of a Western Blot

question 24

Multiple Choice

Below are the steps of a Western blot.What is the correct order?
1) The membrane is incubated with the secondary antibody.
2) The membrane is incubated with the primary antibody.
3) The membrane is washed with a blocking solution.
4) Proteins are transferred to a nitrocellulose membrane.
5) Protein extracts are separated by gel electrophoresis.

• A)5,4,3,2,1
• B)4,5,2,3,1
• C)3,4,5,1,2
• D)2,5,4,3,1

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Definitions:

Lymphatic System

A network of tissues, organs, and vessels that helps to maintain fluid balance and protect the body from infection.

B-lymphocytes

A type of white blood cell that plays a significant role in the humoral immunity component of the adaptive immune system.

Thymus

A small organ located in the upper chest under the sternum, where T-lymphocytes (T cells) mature and differentiate.

Blood Plasma

The liquid component of blood that carries blood cells, nutrients, hormones, and waste products, and is composed of water, proteins, and dissolved substances.