Below Are the Steps Involved in Cloning Gene Sequences Using

question 98

Multiple Choice

Below are the steps involved in cloning gene sequences using mRNA. Arrange the steps in the appropriate order. 1. The double-stranded cDNA is treated with a restriction endonuclease to generate compatible ends for annealing and ligation.
2) mRNA is isolated from the cell and converted back into double-stranded sequences using reverse transcriptase to generate complementary DNA.
3) The RNA-DNA hybrid is treated with a nuclease to cleave the RNA strand, producing RNA fragments.
4) Reverse transcriptase completes the single-stranded cDNA when it reaches the 5 ^' end of the mRNA transcript.
5) RNA fragments serve as primers for DNA synthesis of the second strand of cDNA using DNA polymerase.

• A) 4, 2, 1, 5, 3
• B) 2, 4, 3, 5, 1
• C) 2, 3, 5, 4, 1
• D) 3, 2, 5, 4, 1

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Definitions:

Cumulative Frequency

The sum of frequencies accumulated up to a certain point in a data set.

Total Number

The aggregate or sum count of items or entities in a set or dataset.

Data Set

A collection of data, often presented in tabular form, consisting of variables and observed or measured values.

Class Width

The difference between the upper and lower boundaries of any class (or category) in a frequency distribution.