You Isolate an Infectious Substance That Is Capable of Causing

question 35

Multiple Choice

You isolate an infectious substance that is capable of causing disease in plants,but you do not know whether the infectious agent is a bacterium,virus,or prion.You have four methods at your disposal that you can use to analyze the substance in order to determine the nature of the infectious agent.
I.Treat the substance with nucleases that destroy all nucleic acids and then determine whether it is still infectious.
II.Filter the substance to remove all elements smaller than what can be easily seen under a light microscope.
III.Culture the substance by itself on nutritive medium,away from any plant cells.
IV.Treat the sample with proteases that digest all proteins and then determine whether it is still infectious.
If you already knew that the infectious agent was a prion,which treatment listed above would allow you to distinguish this?

• A)I only
• B)II only
• C)III only
• D)either I or IV

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