For a Science Fair Project,two Students Decided to Repeat the Hershey

question 62

Multiple Choice

For a science fair project,two students decided to repeat the Hershey and Chase experiment,with modifications.They decided to label the nitrogen of the DNA rather than the phosphate.They reasoned that each nucleotide has only one phosphate and two to five nitrogens.Thus,labeling the nitrogens would provide a stronger radioactive signal than labeling the phosphates.Why won't this experiment work?

• A)There is no radioactive isotope of nitrogen.
• B)The phosphate in normal DNA is naturally radioactive.
• C)Although there are more nitrogens in a nucleotide,labeled phosphates actually have 16 extra neutrons;therefore,they are more radioactive.
• D)Amino acids (and thus proteins)also have nitrogen atoms;thus,the radioactivity would not distinguish between DNA and proteins.

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