What Is the Most Logical Sequence of Steps for Splicing

question 31

Multiple Choice

What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium?

• A)Transform bacteria with a recombinant DNA molecule.Cut the plasmid DNA using restriction enzymes.Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments.Extract plasmid DNA from bacterial cells.Use ligase to seal plasmid DNA to nonplasmid DNA.
• B)Cut the plasmid DNA using restriction enzymes.Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments.Use ligase to seal plasmid DNA to nonplasmid DNA.Extract plasmid DNA from bacterial cells.Transform bacteria with a recombinant DNA molecule.
• C)Extract plasmid DNA from bacterial cells.Cut the plasmid DNA using restriction enzymes.Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments.Use ligase to seal plasmid DNA to nonplasmid DNA.Transform bacteria with a recombinant DNA molecule.
• D)Extract plasmid DNA from bacterial cells.Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments.Use ligase to seal plasmid DNA to nonplasmid DNA.Transform bacteria with a recombinant DNA molecule.Cut the plasmid DNA using restriction enzymes.

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