You Prepare Bacterial Cell Extracts by Lysing the Cells and Removing

question 36

Multiple Choice

You prepare bacterial cell extracts by lysing the cells and removing insoluble debris via centrifugation.These extracts provide the proteins required for DNA replication.Your DNA template is a small, double-stranded circular piece of DNA (a plasmid) with a single origin of replication and a single replication termination site.The termination site is on the opposite side of the plasmid from the origin. Figure 6-11
Which of the following statements is TRUE with respect to this in vitro replication system?

• A)There will be only one leading strand and one lagging strand produced using this template.
• B)The leading and lagging strands compose one half of each newly synthesized DNA strand.
• C)The DNA replication machinery can assemble at multiple places on this plasmid.
• D)One daughter DNA molecule will be slightly shorter than the other.

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