You Prepare Bacterial Cell Extracts by Lysing the Cells and Removing

question 50

Multiple Choice

You prepare bacterial cell extracts by lysing the cells and removing insoluble debris via centrifugation.These extracts provide the proteins required for DNA replication.Your DNA template is a small, double-stranded circular piece of DNA (a plasmid) with a single origin of replication and a single replication termination site.The termination site is on the opposite side of the plasmid from the origin. Figure 6-11
What part of the DNA replication process would be most directly affected if a strain of bacteria lacking the exonuclease activity of DNA polymerase were used to make the cell extracts?

• A)initiation of DNA synthesis
• B)Okazaki fragment synthesis
• C)leading-strand elongation
• D)lagging-strand completion

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Definitions:

Extended Family

A family group that extends beyond the immediate nuclear family, including grandparents, aunts, uncles, and other relatives.

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Macro Family

A larger grouping of languages that are believed to be genetically related, encompassing smaller language families under a shared ancestry.

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A family that has unique needs or circumstances, often related to healthcare, developmental, or educational considerations.