Why Is an Excess of Normal Deoxyribonucleoside Triphosphate Molecules (DNTPs)

question 14

Multiple Choice

Why is an excess of normal deoxyribonucleoside triphosphate molecules (dNTPs) needed during dideoxy sequencing?

• A)DNA polymerase uses the dNTPs to synthesize a DNA molecule complementary to the molecule being sequenced.
• B)dNTPs are consumed as energy to fuel the sequencing reactions.
• C)When dNTP levels are too low, there will be very few chain-termination events.
• D)The dNTPs can hybridize to the fragment to be sequenced and serve as primers for DNA polymerase.

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