Figure 10-13 Depicts a Strategy by Which a DNA Fragment

question 15

Multiple Choice

Figure 10-13 depicts a strategy by which a DNA fragment produced by cutting with the EcoRI restriction nuclease can be joined to a DNA fragment produced by cutting DNA with the HaeIII restriction nuclease. Figure 10-13
Note that cutting DNA with EcoRI produces a staggered end, whereas cutting DNA with HaeIII produces a blunt end.Why must polymerase be added in this reaction?

• A)Polymerase will fill in the staggered end to create a blunt end.
• B)Polymerase is needed to seal nicks in the DNA backbone.
• C)Polymerase will add nucleotides to the end produced by the HaeIII restriction nuclease.
• D)Without polymerase, there will not be enough energy for the reaction to proceed.

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