You Want to Design a Set of PCR Primers to Specifically

question 11

Multiple Choice

You want to design a set of PCR primers to specifically amplify a portion of a gene from genomic DNA.Which of the following statements about this set of primers is TRUE?

• A)One of the primers should contain sequences complementary to common repetitive sequences in the genome, to ensure that it will hybridize to the genomic DNA.
• B)Each primer should span the junction of two adjacent exons and leave out intron sequences, to ensure that a portion of the coding portion of the gene is amplified and not noncoding regulatory sequence.
• C)If the primers include sequence from both an exon and the adjacent intron, this will help ensure that the amplified product is made from genomic DNA and not any contaminating mRNA.
• D)The primers should use "U"s instead of "T"s to ensure that the coding portion of the gene is amplified.

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Definitions:

Market Share

The proportion of the total sales or output produced in a market by a single company, often used as an indicator of competitiveness.

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A field of economics that examines how psychological, cognitive, emotional, cultural, and social factors affect economic decisions of individuals and institutions.

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Fees paid, usually on a monthly or annual basis, for access to a health club or gym's facilities and services.

Horizontal Differentiation

A market strategy where products are made to appear different from competitors' products in ways other than price, such as through branding, packaging, or quality variations.