Cell Lines a and B Both Survive in Tissue Culture

question 50

Multiple Choice

Cell lines A and B both survive in tissue culture containing serum but do not proliferate.Factor F is known to stimulate proliferation in cell line A.Cell line A produces a receptor protein (R) that cell line B does not produce.To test the role of receptor R, you introduce this receptor protein into cell line B, using recombinant DNA techniques.You then test all of your various cell lines in the presence of serum for their response to factor F, with the results summarized in Table 16-9. $\begin{array} { l l l } \text { Cell line } & \text { Factor } F & \text { Response } \\\hline \text { A } & \text { - Factor F } & \text { Cells do not proliferate } \\\text { A } & \text { + Factor F } & \text { Cells proliferate } \\\text { B } & \text { - Factor F } & \text { Cells do not proliferate } \\\text { B } & \text { + Factor F } & \text { Cells do not proliferate } \\\text { B + receptor R } & \text { - Factor } F & \text { Cells proliferate } \\\text { B + receptor R } & \text { + Factor F } & \text { Cells proliferate }\end{array}$ Table 16-9
Which of the following cannot be concluded from your results above?

• A)Binding of factor F to its receptor is required for proliferation of cell line A.
• B)Receptor R binds to factor F to induce cell proliferation in cell line A.
• C)Cell line A expresses a receptor for factor F.
• D)Factor F is not required for proliferation in cell line B.

---

Definitions:

Tax Considerations

Refers to the factors related to taxes that need to be taken into account when making financial or business decisions.

Liability

A legal obligation or responsibility for the consequences of one's actions, potentially resulting in payment or other remedies in a legal context.

Capital

Financial assets or resources that individuals or organizations use to fund their operations and invest in their growth.

Competitors' Organization

A structured group or business that competes in the same industry or market as another business.