You Are Interested in Understanding the Regulation of Nuclear Lamina

question 56

Multiple Choice

You are interested in understanding the regulation of nuclear lamina assembly.To create an in vitro system for studying this process you start with partly purified nuclear lamina subunits to which you will add back purified cellular components to drive nuclear lamina assembly.Before you start doing experiments, your instructor suggests that you consider what type of conditions would be most amenable to the assembly of the nuclear lamina from its individual subunits in vitro.Which of the following additions do you predict would be most likely to enhance the assembly of the nuclear lamina?

• A)addition of phosphatase inhibitors
• B)addition of ATP
• C)addition of a concentrated salt solution that is 10 times the concentration normally found in the nucleoplasm
• D)addition of protein kinase inhibitors

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