In a FISH Experiment, What Would Happen If Unbound Probe

question 43

Multiple Choice

In a FISH experiment, what would happen if unbound probe was not washed off?

• A) Nothing-it's not necessary to wash off the unbound probe and doing so just adds an extra step to the procedure.
• B) You would get false-positive results in different areas where the probe hadn't actually bound, but it was still sitting there and lighting up.
• C) Your FISH would be floating at the top of the tank due to the toxicity of the probe building up within them.
• D) Nothing-the target nucleotide sequences are labeled, not the probe. Therefore, excess unbound probe wouldn't matter for the experiment.
• E) You would get an intermediate color on the array because of the presence of both bound and unbound probes that fluoresce at different temperatures.

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