Why Does a Genomic Library Need More Clones Than What

question 24

Multiple Choice

Why does a genomic library need more clones than what would be estimated by calculations to be complete?

• A)There is a significant probability that some sequences would not be represented in a library if it was only the calculated size.
• B)The restriction enzyme that is used to prepare the DNA for cloning will not digest certain sequences within the DNA.
• C)The restriction enzyme used to prepare the DNA for cloning sometimes leaves a different sticky end.
• D)Restriction enzymes cleave DNA preferentially at the ends of open reading frames so coding regions are preferentially cloned.

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