Use the Following to Answer Questions
You Are Studying Epigenetic

question 44

Multiple Choice

Use the following to answer questions
You are studying epigenetic regulation of the Bdnf gene in mice and its association with memory. You train mouse B to avoid an adverse stimulus and then isolate genomic DNA from its brain. Mouse A is untrained and used as a control. You take the brain DNA samples and split each of them into two parts. One part is treated with sodium bisulfite and the other part is left untreated. You then perform PCR for the Bdnf locus and digest the PCR product with the restriction enzyme AccII that cuts DNA at the sequence CGCG. The digested DNA is then separated by agarose gel electrophoresis to obtain the following DNA fragments:
$$\begin{array}{l}\begin{array} { c c } \text { Mouse A (Bisulfite DNA) } & \text { Mouse A (Untreated DNA) } \\\hline 21.2 \mathrm {~kb} & 19.3 \mathrm {~kb} \\7.4 \mathrm {~kb} & 7.4 \mathrm {~kb} \\5.8 \mathrm {~kb} & 5.1 \mathrm {~kb} \\5.7 \mathrm {~kb} & 3.9 \mathrm {~kb} \\4.9 \mathrm {~kb} & 3.5 \mathrm {~kb} \\3.5 \mathrm {~kb} & 2.7 \mathrm {~kb} \\& 2.2 \mathrm {~kb} \\& 1.9 \mathrm {~kb} \\& 1.8 \mathrm {~kb} \\& 0.7 \mathrm {~kb}\end{array}\\\\\begin{array} { c c } \text { Mouse B (Bisulfite DNA) } & \text { Mouse B (Untreated DNA) } \\\hline 19.3 \mathrm {~kb} & 19.3 \mathrm {~kb} \\7.4 \mathrm {~kb} & 7.4 \mathrm {~kb} \\5.7 \mathrm {~kb} & 5.1 \mathrm {~kb} \\5.1 \mathrm {~kb} & 3.9 \mathrm {~kb} \\4.9 \mathrm {~kb} & 3.5 \mathrm {~kb} \\3.5 \mathrm {~kb} & 2.7 \mathrm {~kb} \\1.9 \mathrm {~kb} & 2.2 \mathrm {~kb} \\0.7 \mathrm {~kb} & 1.9 \mathrm {~kb} \\& 1.8 \mathrm {~kb} \\& 0.7 \mathrm {~kb}\end{array}\end{array}$$
-How many of the AccII sites were methylated at the Bdnf locus in the untrained mouse?

• A) 1
• B) 2
• C) 3
• D) 4
• E) 5

---

Definitions:

Self-determination Theory

A theory of human motivation that emphasizes the role of intrinsic motivation in driving human behavior and the importance of psychological needs for autonomy, competence, and relatedness.

Stressor

Any event, situation, or stimulus that causes stress, challenging an individual's ability to cope and potentially impacting mental and physical health.

Hassles

The daily annoyances of everyday life.

Hassles

Minor irritations or annoyances that occur in daily life and can accumulate to cause stress.