Use the Following to Answer Questions

question 23

Essay

Use the following to answer questions
Figure A below shows a restriction map of a rare prokaryotic gene with its direction of transcription indicated by the arrow. Figure B shows the unique restriction sites contained within a plasmid-cloning vector. The blackened region in Figure A represents the amino acid coding sequence of a protein that can be used in humans as a vaccine. The striped region in Figure B is a highly active, constitutive (unregulated) prokaryotic promoter region. Letters indicate the cleavage sites for different restriction enzymes. Known DNA sequences are indicated by short thick lines.
-Explain how you would isolate and then insert the coding region (Figure
A) under the control of the indicated promoter in the cloning vector (Figure
B) to produce large amounts of the protein in bacterial cells. Assume that the cloning vector carries the gene for tetracycline (an antibiotic) resistance.

• A) under the control of the indicated promoter in the cloning vector (Figure
• B) to produce large amounts of the protein in bacterial cells. Assume that the cloning vector carries the gene for tetracycline (an antibiotic) resistance.

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