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You are studying epigenetic regulation of the Bdnf gene in mice and its association with memory. You train mouse B to avoid an adverse stimulus and then isolate genomic DNA from its brain. Mouse A is untrained and used as a control. You take the brain DNA samples and split each of them into two parts. One part is treated with sodium bisulfite and the other part is left untreated. You then perform PCR for the Bdnf locus and digest the PCR product with the restriction enzyme AccII that cuts DNA at the sequence CGCG. The digested DNA is then separated by agarose gel electrophoresis to obtain the following DNA fragments:
-How many of the AccII sites were methylated at the Bdnf locus in the untrained mouse?
Culture
The collective intellectual achievements, arts, and social institutions of a particular nation or social group.
Letter Of Refusal
A formal written communication to decline an offer, invitation, application, or request, typically providing reasons for the refusal.
Available Choices
The range of options or alternatives that an individual can select from at any given time.
Negative Message
Communication that conveys a refusal, rejection, or unfavorable information, often requiring careful wording to maintain goodwill.
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