Below Are the Steps Involved in the RNA-Seq Process

question 66

Multiple Choice

Below are the steps involved in the RNA-seq process. Place these steps in the CORRECT order. 1. Conversion of the RNA to cDNA
2) Fragmentation and preparation of the cDNAs for sequencing
3) Isolation of the RNA molecules of interest from cells
4) Assembly of the sequencing reads into RNA transcripts
5) Sequencing of the cDNA

• A) 3, 1, 2, 5, 4
• B) 1, 2, 4, 5, 3
• C) 3, 4, 1, 2, 5
• D) 1, 5, 4, 3, 2
• E) 1, 5, 2, 3, 4

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Definitions:

Dinoflagellate

A group of single-celled, mostly marine organisms with two flagella, known for causing bioluminescence and red tides.

Euglenoid

Free-living, flagellated protist in the supergroup Excavata; some have chloroplasts descended from a green alga.

Amoeba

Single-celled protist that extends pseudopods to move and to capture prey.

Mantle

Of mollusks, a skirt-like extension of the body wall.