Use the Following to Answer Questions

question 44

Multiple Choice

Use the following to answer questions
A student carries out PCR using the following steps:
Step 1: 94°C for 1 minute
Step 2: 60°C for 30 seconds
Step 3: 72°C for 30 seconds
-Which of the following lists the CORRECT terms for these three steps?

• A) denaturation of the double-stranded template, extension of the new DNA molecules, primer annealing
• B) denaturation of the double-stranded template, primer annealing, extension of the new DNA molecules
• C) denaturation of the double-stranded template, extension of the new DNA molecules, hybridization of the template
• D) degradation of the template, primer annealing, extension of the new DNA molecules
• E) hybridization of the single-stranded templates, primer annealing, extension of the new DNA molecules

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