Eukaryotic Genes Can Be Introduced into Bacteria by Recombinant DNA

question 34

Essay

Eukaryotic genes can be introduced into bacteria by recombinant DNA techniques. If the introduced gene encodes a protein that is also found in bacteria-for example, a universally used glycolysis enzyme-then expression of the eukaryotic gene may produce a protein that functions in the bacterial cell.
The mouse gene for a glycolysis enzyme is introduced into an E. coli cell that has a mutant gene for the bacterial version of the same enzyme. Even though the mouse enzyme should function in the bacterial cell and restore the cell's ability to perform glycolysis, it does not.
Provide two possible reasons why this experiment does not work and propose a solution to overcome one of the problems you suggest.

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Definitions:

Adjusted Trial Balance

A statement listing all accounts and their final balances after adjusting entries are made, used to prepare financial statements.

Closing Entries

Journal entries made at the end of an accounting period to transfer the balances of temporary accounts to permanent ones.

Retained Earnings Account

An equity account that represents the accumulated portion of net income that a company retains after distributing dividends to shareholders.

Balance Sheet Accounts

Categories in a balance sheet including assets, liabilities, and shareholders' equity, representing a company's financial position.