Use the Following to Answer Questions

question 57

Multiple Choice

Use the following to answer questions
A student carries out PCR using the following steps:
Step 1: 94°C for 1 minute
Step 2: 60°C for 30 seconds
Step 3: 72°C for 30 seconds
-After electrophoresing his PCR reaction, the student sees the desired PCR product on the gel as well as several smaller bands. What error(s) might he have made? (Select all that apply.)

• A) He carried out step 2 at too low a temperature
• B) He carried out step 2 at too high a temperature.
• C) He designed primers with repetitive sequences.
• D) He contaminated the template DNA sample.
• E) He carried out step 3 for too short a time.

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Definitions:

Avoidance Motivation

Worrying about the negative consequences of failure.

Safety And Security

Fundamental needs referring to the protection from harm and assurance of physiological and psychological wellbeing.

Humanistic Personality

A psychological perspective that emphasizes individual growth, self-actualization, and the concept of the self, viewing individuals as inherently good with potential for personal development.

Self-Understanding

An individual's awareness and comprehension of their own personality, behaviors, emotions, motivations, and beliefs.