You Have Discovered a Gene That Enables Organisms to Accumulate

question 55

Multiple Choice

You have discovered a gene that enables organisms to accumulate gold in their tissues by concentrating trace amounts found in normal soil. You want to transfer this gene into a plant. Order the steps below that would accomplish this goal. 1. Infect the plant with the Agrobacterium strain.
2) Digest the gold gene and a Ti plasmid with appropriate restriction enzymes.
3) Insert the gold gene into the Ti plasmid.
4) Amplify the gold gene with PCR.
5) Transfer the recombinant Ti plasmid into Agrobacterium tumefaciens.
6) Use a selectable marker to identify plant cells that have integrated the recombinant plasmid into their genome.

• A) 4, 2, 3, 5, 1, 6
• B) 4, 5, 2, 3, 1, 6
• C) 4, 2, 5, 1, 6, 3
• D) 4, 3, 2, 5, 1, 6

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