You Are Trying to Determine Whether a Certain RFLP Marker

question 35

Not Answered

You are trying to determine whether a certain RFLP marker is linked to a specific disease gene in dogs. A cross between a diseased male and a healthy female produces nine offspring. You isolate genomic DNA from parents and offspring, digest with EcoRI, radiolabel a portion of RFLP marker to use as a probe, and perform a Southern analysis. The pedigree and autoradiogram results are shown in the following figure. Assume that the disease is autosomal dominant. (a) Explain the relationship between the crossing results (i.e., diseased versus healthy) and the RFLP patterns.

---

Definitions:

Overhead Volume Variance

The difference between the budgeted overhead based on standard hours allowed and the actual overhead incurred, due to differences in activity levels.

Unfavorable Labor Efficiency Variance

A financial term indicating that the actual labor time to produce goods or services was higher than the budgeted or standard labor time, resulting in increased costs.

Variance Report

A document that compares planned financial outcomes to actual financial outcomes, highlighting differences (variances) for analysis.

Materials Costs

These are the costs associated with the raw materials used in the manufacture of products or the provision of services.