An in Vitro Transcription System Transcribes a Bacterial Gene but Terminates

question 65

Multiple Choice

An in vitro transcription system transcribes a bacterial gene but terminates inefficiently. What is one possible problem?

• A) There is a mutation in the -10 consensus sequence, which is required for efficient termination.
• B) Rho factor has not been added.
• C) Sigma factor has not been added.
• D) A hairpin secondary structure has formed at the 3' end of the mRNA, interfering with termination.
• E) Histones were added prematurely and interfered with termination.

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Definitions:

Mediation

A form of alternative dispute resolution where a neutral third party helps those in conflict find a mutually acceptable solution.

Progressive Discipline

A process by which an employer provides a series of warnings or disciplinary actions to an employee for violating company policies or performance standards, with the goal of improving behavior or performance.

Termination Offence

An action or behavior by an employee that is considered severe enough to justify immediate dismissal from their position.

Guilty

The legal status of a person who has been convicted of a crime or misdemeanor, proving their responsibility for the offense.