The Three Basic Steps of Polymerase Chain Reaction (PCR)are Denaturation

question 44

Multiple Choice

The three basic steps of polymerase chain reaction (PCR) are denaturation, primer annealing, and primer extension. Which specification listed below is correct?

• A)Primer design-oligonucleotide of 15- 30 base pairs
• B)Primer extension-10 minutes during each cycle
• C)Primer annealing-95° C for 1 minute
• D)Denaturation-50° C for 5 minutes
• E)Primer extension-37° C when Taq polymerase is used

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