Which of the Following Places the Steps in the PCR

question 15

Multiple Choice

Which of the following places the steps in the PCR procedure in the correct order?
1) Incubate at 94°C to denature DNA strands;
2) Incubate at 72°C for DNA synthesis;
3) Incubate at 60°C for primer hybridization.

• A) 2; 1; 3
• B) 1, 2, 3
• C) 3, 2, 1
• D) 1, 3, 2
• E) 3; 1; 2

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Definitions:

FTA-ABS

Fluorescent treponemal antibody absorption, a blood test used for detecting antibodies to the bacterium Treponema pallidum, the causative agent of syphilis.

Syphilis

A sexually transmitted infection caused by the bacterium Treponema pallidum, characterized by distinct stages and potentially serious long-term symptoms if untreated.

Ejaculation

The expulsion of semen from the body during sexual climax.

Human Papillomavirus

A group of viruses, some of which can cause genital warts or lead to certain cancers, including cervical cancer.