To Map Genes of a Bacterial Strain, Conjugation Must Be

question 35

Multiple Choice

To map genes of a bacterial strain, conjugation must be interrupted at given times. Suppose you have Hfr cells of genotype a+b+c+d+e+strR and F- cells of genotype a- b- c- d- e- strS and you combine these two cultures in liquid medium in four blenders at time 0. After intervals of 3, 6, 9, and 12 minutes, you turn on successive blenders. What role does the blender play in the experiment?

• A) shears the sex pili of conjugants
• B) serves as a culture container only
• C) acts as a mechanism to bring cells into contact
• D) hastens conjugation events
• E) speeds up molecular movement of DNA

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