A Researcher Is Annotating a Newly- Sequenced Chromosome That They

question 5

Multiple Choice

A researcher is annotating a newly- sequenced chromosome that they constructed from contigs sequenced from a sample of Antarctic ice. The cell/organism that the chromosome came from is not known, however it yielded a single circular chromosome. You are asked to run a BLAST search to help this researcher identify the most related organism in GenBank. What would be the best strategy?

• A) Perform many careful one- by- one comparisons between the newly- discovered DNA sequence and species selected from GenBank, one at a time.
• B) Look for an exact match to the whole chromosome.
• C) Look for a nearly identical match to a very small part (20- 40 bp) of the chromosome from all known ice bacteria.
• D) Use several ~1000bp portions of the DNA sequence to search for the closest matches from among the entire available database.
• E) Compare the most unusual portion of this newly- discovered chromosome to al known animal genomes.

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Definitions:

All Equity

A capital structure of a company or financial strategy where all financing is derived from shareholders' equity rather than combining equity with debt financing.

Leverage

Utilizing borrowed capital to amplify the possible gains from an investment.

Interest Rate

The percentage of a sum of money charged for its use, typically expressed on an annual basis.

All Equity

A financing structure where a company's capital structure is composed entirely of equity without any debt.