Which of the Following Lists the Steps for CDNA Library

question 50

Multiple Choice

Which of the following lists the steps for cDNA library preparation in the correct order? I. Partially degrade the mRNA with RNase H
II. Isolate mRNA from a specific cell/ tissue with an oligo- dT primer that hybridizes to poly- A tail
III. Synthesize a strand of cDNA using DNA polymerase with mRNA fragments as primers
IV. Ligate cDNAs into a cloning vector
V. Create a single- stranded DNA molecule complementary to the mRNA using reverse transcriptase

• A) II, V, IV, III, I
• B) II, V, I, III, IV
• C) I, II, III, IV, V
• D) II, III, V, I, IV
• E) V, III, I, IV, II

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Definitions:

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The voltage difference across the cell membrane of a neuron at rest, typically around -70mV.

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A long, slender projection of a nerve cell that conducts electrical impulses away from the neuron's cell body.

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The occipital lobe is the cerebral cortex region in the back of the brain, responsible for processing visual information from the eyes.