Which of the Following Lists the Steps for CDNA Library

question 50

Multiple Choice

Which of the following lists the steps for cDNA library preparation in the correct order? I. Partially degrade the mRNA with RNase H
II. Isolate mRNA from a specific cell/ tissue with an oligo- dT primer that hybridizes to poly- A tail
III. Synthesize a strand of cDNA using DNA polymerase with mRNA fragments as primers
IV. Ligate cDNAs into a cloning vector
V. Create a single- stranded DNA molecule complementary to the mRNA using reverse transcriptase

• A) II, V, IV, III, I
• B) II, V, I, III, IV
• C) I, II, III, IV, V
• D) II, III, V, I, IV
• E) V, III, I, IV, II

Recognize how text-based messages can limit emotional expression according to specific communication theories.

Explain the social information processing theory and its implications for conveying relational and emotional messages electronically.

Understand how both content and relationship dimensions influence interpersonal communication.

Identify behaviors that indicate a lack of consideration for the listener in communication processes.

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Definitions:

Technological

Pertaining to technology, involving or employing technology in a product, service, or method of operation.

Commercial

Relating to business activities, transactions, or advertising intended to generate revenue.

Environmental Forces

External factors, including social, economic, technological, competitive, and regulatory forces, that can affect an organization's operations and business decisions.

Marketing Activities

Actions undertaken by a company to promote and sell its products or services, including advertising, sales promotions, and public relations.