In Order to Identify Genes That Function Together in Genetic

question 7

Multiple Choice

In order to identify genes that function together in genetic pathways researchers can perform modifier screens, looking for second site enhancers or suppressors of a mutant. Which of the following is the most reasonable strategy for carrying out a suppressor screen in particular?

• A) screen for enhancer- trap strains that show gene expression differences in the original mutant.
• B) mutagenize the original mutant and look for new mutations that reduce the severity of the original mutant phenotype
• C) isolate mutants with phenotypes similar to the original mutant phenotype and then cross the mutants to identify the affected gene(s)
• D) identify mutations that, when crossed with the original mutant, result in synthetic lethality
• E) analyze the DNA sequence near the mutation of interest, looking for sequences that may act as silencers

Identify the role of intuition, authority, and skepticism in scientific research.

Recognize the importance of empiricism and falsifiability in the scientific method.

Be aware of the process and significance of peer review in scientific research.

Appreciate the ethical considerations in conducting and evaluating scientific research.

---

Definitions:

Ecological Problem

Issues that arise when the natural balance of ecosystems is disturbed, leading to environmental harm and decreased biodiversity.

Industrial Smog

A type of air pollution generated from industrial operations and fossil fuel combustion, characterized by a mix of particulates and gases.

Sulphur Dioxide

A toxic gas with a pungent, irritating smell, commonly resulting from the burning of fossil fuels and is a significant pollutant in the atmosphere.

Sulphuric Acid

A highly corrosive strong mineral acid with the molecular formula H2SO4, used in a wide variety of industrial processes.