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In TILLING, a Population of Inbred Organisms Is Mutagenized to Saturation

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In TILLING, a population of inbred organisms is mutagenized to saturation. DNA from mutagenized lines is isolated and amplified by PCR. PCR products are denatured and allowed to reanneal and then treated with an endonuclease. Why is the denaturation/re- annealing step important for the detection of mutant alleles?


Definitions:

Distortion

A modification of the original shape or other characteristic of an object, image, sound, or waveform.

Eyewitness Memory

The recollection by individuals of events they have witnessed, especially significant to criminal investigations.

Laboratory Experiments

Controlled tests or investigations conducted in a specially equipped space where variables can be manipulated and measured to explore scientific theories.

Real-World Stimuli

External inputs from the environment that can be perceived and interacted with in everyday life.

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