In TILLING, a Population of Inbred Organisms Is Mutagenized to Saturation

question 21

Essay

In TILLING, a population of inbred organisms is mutagenized to saturation. DNA from mutagenized lines is isolated and amplified by PCR. PCR products are denatured and allowed to reanneal and then treated with an endonuclease. Why is the denaturation/re- annealing step important for the detection of mutant alleles?

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