In the Process of Isolating DNA for Transcription and Translation

question 4

Multiple Choice

In the process of isolating DNA for transcription and translation, you digest the DNA with BamHI and ligate the gene into a plasmid vector. However, even though all of the controls work, no transcription is obtained using your fragment. Working backward, you note that restriction digestion has removed approximately 250 base pairs from the 5' end of the gene. It is possible that during restriction digestion

• A)the promoter was removed.
• B)the RNA polymerase became inactivated.
• C)the termination signal was removed.
• D)primase could not bind.
• E)none of the above

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Definitions:

Physical Factors

Environmental elements that can influence a person's health, activities, or the functioning of ecosystems, such as climate, geography, and pollution.

Room Temperature

The ambient temperature of a living space, considered comfortable for most humans, usually around 20-22°C (68-72°F).

Defense Mechanism

Psychological strategies brought into play by individuals, unconsciously, to protect themselves from anxiety or to confront uncomfortable emotions.

Unpleasant Truth

A fact or reality that is difficult to accept or confront due to its distressing, uncomfortable, or disagreeable nature.