Using Restriction Enzymes, a Eukaryotic Gene Is Cut, Ligated into a Bacterial

question 1

Multiple Choice

Using restriction enzymes, a eukaryotic gene is cut, ligated into a bacterial plasmid, and introduced into a strain of E. coli. To better the odds of getting expression of the eukaryotic gene in this system, you need to make certain that

• A)the gene and the plasmid were cut with different restriction enzymes.
• B)the gene has a eukaryotic promoter for transcription.
• C)mRNA is used for ligation directly into the vector.
• D)the plasmid has a prokaryotic promoter for transcription.
• E)the gene product is toxic to the bacterial cell.

---

Definitions:

Implied Contract

An agreement created by actions of the parties involved rather than written or spoken words, indicating an unexpressed understanding or assumption of agreement.

Quasi-contract

A legal concept where a court imposes an obligation on a party to prevent unjust enrichment, even though no actual contract exists.

Void Contract

A contract that has no legal effect and is unenforceable from its inception due to certain legal deficiencies or violations.

Unenforceable Contract

An agreement that is not legally binding, either due to its failure to meet certain legal requirements or because it involves illegal activities.