You Isolate a New Bacterium from Soil with a Revolutionary

question 18

Multiple Choice

You isolate a new bacterium from soil with a revolutionary new growth technique. You are interested in characterizing the genome of this organism using restriction enzymes. The first enzyme chosen should be one that will cut the genome infrequently, allowing you to do more detailed fine mapping at a later date. Of the following choices, the best enzyme to choose for your task would be (NOTE: P = a purine base; Y = a pyrimidine base)

• A)TaqI (TCGA).
• B)HindII (GTYPAC).
• C)NotI (GCGGCCGC).
• D)HpaI (CCGG).
• E)EcoRI (GAATTC).

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Definitions:

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